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Naukowcy z Biobanku Zakładu Medycznej Diagnostyki Laboratoryjnej Gdańskiego Uniwersytetu Medycznego wchodzącego w skład konsorcjum BBMRI.pl we współpracy z Beckman Institute of Advanced Science, University of Illinois at Urbana-Champaign oraz Uniwersytetem Medycznym w Łodzi opublikowali artykuł "Evaluation of a dimeric-cRGD peptide for targeted PET-CT imaging of peripheral angiogenesis in diabetic mice". Zespoły oceniały dimeryczny peptyd cRGD znakowany 64Cu wiążący się z integryną α V β3 w kontekście jego przydatności do badań obwodowej angiogenezy w cukrzycy typu I. Udowodniono znaczącą redukcję angiogenezy z zastosowaniem PET-CT u myszy cukrzycowych. Artykuł ukazał się w marcowym numerze Scientific Reports (IF = 4.609).  Zapraszamy do lektury.

 

 

Jamila Hedhli1,2, Stephanie L. L. Slania2, Agata Płoska1,3, Andrzej Czerwinski4, Christian J. Konopka1,2, Marcin Wozniak1,3, Maciej Banach5, Iwona T. Dobrucki1, Leszek Kalinowski3,6 and Lawrence W. Dobrucki1,2,3,6

Beckman Institute for Advanced Science and Technology, Urbana, IL USA
Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, IL USA
Department of Medical Laboratory Diagnostics and Central Bank of Frozen Tissues & Genetic Specimens, Medical University of Gdansk, Gdansk, Poland
Peptides International Inc, Louisville, KY USA
Department of Hypertension, Medical University of Lodz, Lodz, Poland
Biobanking and Biomolecular Resources Research Infrastructure Poland (BBMRI.PL), Gdansk, Poland

 

 Abstract

The α V β3 integrin plays an important role in many physiological functions and pathological disorders. α V β3 is minimally expressed in normal quiescent endothelial cells, but significantly upregulated during neovascularization. In this study, we evaluated a 64Cu-labeled dimeric cRGD tracer targeted at α V β3 integrin and report its applicability to assess peripheral angiogenesis in diabetes mellitus (DM). We established a murine model of type-1 DM characterized by elevated glucose, glycated serum protein (GSP), and glycated hemoglobin A1c (HbA1c). We demonstrated that our imaging probe is specific to α V β3integrin under both normo- and hyperglycemic conditions. We found that the analysis of in vivo PET-CT images correlated well with gamma well counting (GWC). Both GWC and PET-CT imaging demonstrated increased uptake of 64Cu-NOTA-PEG4-cRGD2 in the ischemic hindlimb in contrast to non-ischemic control. GWC of the distal ischemic tissue from DM mice showed significantly lower probe accumulation than in non-DM mice. The immunofluorescence staining of the ischemic tissues showed a 3-fold reduction in CD31 and 4-fold reduction in the αV β3 expression in DM vs. non-DM animals. In conclusion, we successfully demonstrated that diabetes-associated reductions in peripheral angiogenesis can be non-invasively detected with PET-CT imaging using targeted dimeric-cRGD probe.