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PPE51 jest zaangażowany w pobieranie disacharydów przez Mycobacterium tuberculosis
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W najnowszym wydaniu czasopisma Cells , MDPI ukazał się artykuł „PPE51 Is Involved in the Uptake of Disaccharides by Mycobacterium tuberculosis„ w którym naukowcy ukazują zaangażowanie białka PPE51 w absorpcję disacharydów przez prątki gruźlicy.  Cells, IF=5.656.

W badaniach brali udział między innymi pracownicy Pracowni Biobank.

Zapraszamy do lektury.

 

 

PPE51 Is Involved in the Uptake of Disaccharides by Mycobacterium tuberculosis

 
 
1Institute of Medical Biology, Polish Academy of Sciences, 93-232 Lodz, Poland
2Biobank Lab, Department of Molecular Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, 90-237 Lodz, Poland
3Department of Anthropology, Faculty of Biology and Environmental Protection, University of Lodz, 90-237 Lodz, Poland
4Department of Immunoparasitology, Faculty of Biology and Environmental Protection, University of Lodz, 90-237 Lodz, Poland
5Department of Pharmaceutical Sciences, Nesbitt School of Pharmacy, Wilkes University, Wilkes-Barre, PA 18766, USA

 

 
We have recently found that selected thio-disaccharides possess bactericidal effects against Mycobacterium tuberculosis but not against Escherichia coli or Staphylococcus aureus. Here, we selected spontaneous mutants displaying resistance against the investigated thio-glycoside. According to next-generation sequencing, four of six analyzed mutants which were resistant to high concentrations of the tested chemical carried nonsynonymous mutations in the gene encoding the PPE51 protein. The complementation of these mutants with an intact ppe51 gene returned their sensitivity to the wild-type level. The uptake of tritiated thio-glycoside was significantly more abundant in wild-type Mycobacterium tuberculosis compared to the strain carrying the mutated ppe51 gene. The ppe51 mutations or CRISPR-Cas9-mediated downregulation of PPE51 expression affected the growth of mutant strains on minimal media supplemented with disaccharides (maltose or lactose) but not with glycerol or glucose as the sole carbon and energy source. Taking the above into account, we postulate that PPE51 participates in the uptake of disaccharides by tubercle bacilli.